Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Counterparts
A) Transduction of hESCs (WA07 line,) and differentiated cells (H7TF, HFF, and IMR-90 lines) with organelle lights MitoGFP was used investigate mitochondria morphology and localization within the cell. Blue: DAPI; Green: pyruvate dehydrogenase GFP. Scale bar: 10 µm B) Transmission electron microscopy (TEM) was used to investigate mitochondria morphologic features in hESCs (WA07 line), IPSCs (HFF1 and IMR-90 IPSC lines), and fibroblasts cells (H7TF, IMR-90 and HFF1). Scale bar: 500 nm.