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A High-Throughput Screen for Tuberculosis Progression

Figure 3

Pipeline of high-throughput infection of zebrafish embryos and subsequent drug testing.

(A) After fertilization eggs are harvested, washed and distributed on injection plate. (B) Appropriate inoculum is injected in early stage embryos (up to 1,024-cell stage). (C) Injected embryos are dispensed into appropriate containers and drug screens take place between 3 and 6 dpi. (D) Groups of treated and untreated embryos are separately screened using COPAS during (when appropriate) and after drug exposure. Detailed optical analyses are performed on selected larvae.

Figure 3