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Acyl-Protein Thioesterase 2 Catalizes the Deacylation of Peripheral Membrane-Associated GAP-43

Figure 6

Effect of APT-2 overexpression on the deacylation rate of H-RasG12V.

60 h after transient transfection, CHO-K1 cells coexpressing H-RasG12V and APT-2 were treated with 50 µM 2-BP (2-BP) or vehicle (Ctrl) in the presence of CHX and protein degradation inhibitors for 0, 3 and 6 h and H-RasG12V subcellular distribution analyzed by live cell confocal microscopy. Each panel shows image from YFP fluorescence (pseudocolored gray). The insets show cells expressing APT-1 (cherry fluorescence). Scale bar: 5 µm.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0015045.g006