Spirulina Promotes Stem Cell Genesis and Protects against LPS Induced Declines in Neural Stem Cell Proliferation
Human neural progenitors grown under proliferation conditions were assessed by MTT assay (A) or BrdU (B) for the effects of spirulina (125 ng/ml) or NT-020 (500 ng/ml) or the two treatments combined in the presence or absence of TNFα (20 ng/ml) for 72 hours. (A) The MTT assay shows that spirulina alone or NT-020 alone increase proliferation; surprising, the in combination proliferation is decrease compare to control ** p<0.005(compare to media alone). Addition of 20 ng/ml human recombinant TNFα significantly decreased proliferation compare to media alone (***p<0.0001). Only the addition of spirulina alone was able to revert decrease in proliferation †††p<0.05 (compare to TNFα alone). (B) Spirulina and NT-020 alone significantly increased the number of BrdU+ cells. When added together spirulina and NT-020 cause a significant decrease in proliferation ** p<0.005 ***p<0.0001 (compare to media alone). The addition of TNFα significantly decreased proliferation compare to media alone (***p<0.0001). Spirulina alone was able to prevent the decrease in the number of BrdU+ cells. ††† p<0.0001 (compare to TNFα alone).