Revisiting Host Preference in the Mycobacterium tuberculosis Complex: Experimental Infection Shows M. tuberculosis H37Rv to Be Avirulent in Cattle
Blood was collected from cattle following experimental infection with either M. bovis (n = 5) or M. tuberculosis (n = 5). PBMCs were isolated, stimulated with bovine-PPD and the expression of TNF-α measured in the culture supernatant by ELISA. The response in the M. tuberculosis infected cattle is shown as open circles with a dotted line, and for the M. bovis infected cattle as closed triangles with a solid line. Data for each time point is presented as the mean response ± SEM with the no-antigen response subtracted. Significant differences between groups was determined using 2-way ANOVA with Bonferroni post-test analysis (*** p<0.001).