The ADMR Receptor Mediates the Effects of Adrenomedullin on Pancreatic Cancer Cells and on Cells of the Tumor Microenvironment
(A) In vivo targeting of ADMR using DOPC nanoliposome coupled human and mouse siADMR in combination. SiRNAs were delivered (each 10 ug per animal i.p. twice a week) to athymic nude mice bearing human pancreatic cancer cells (MPanc96 with luciferase gene) and monitored for a period of six weeks. These treatments caused a significant reduction in tumor volume as measured by bioluminescence imaging. Representative pictures of mice show the bioluminescence images of treated and untreated pancreatic cancer. (B) Tissues from orthotopic tumors developed with MPanc96 cells and treated with siControl or siADMR (human and mouse in combination) were processed immunohistochemically for CD31 staining. SiControl treated cells showed open blood vessels, while siADMR treated cells showed constricted or collapsed blood vessels. Shown are representative micrographs. (C) Immunohistochemical staining of VEGF showed no differences between siControl or siADMR treated tumors.