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Mechanisms of KGF Mediated Signaling in Pancreatic Duct Cell Proliferation and Differentiation

Figure 4

Induction of D-type cyclins by KGF.

Cell lysates were analyzed by western blot for cyclin D1 and cyclin D2 expression. (A) Cells were cultured in medium with 10% FBS. Basal levels of cyclin D2 were not detectable in Panc-1 cells as compared to its expression in insulinoma cell line, INS-1 used as control for the specificity of antibody. (B) Panc-1 cells were cultured in serum-free medium. Cells were then exposed or not to KGF or PD98059 alone, or to the combination of the two agents for 8 h. Cell lysates were analyzed by western blot for cyclin D1 expression. Results are expressed as mean values ±sem of three independent experiments. * p<0.05.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0004734.g004