A Low Dose of Dietary Resveratrol Partially Mimics Caloric Restriction and Retards Aging Parameters in Mice
Gene expression profiling of 20,687 unique transcripts using Affymetrix Mouse Genome 430 2.0 arrays was performed in young (5-months old) control and old control, CR and resveratrol fed animals (30-months old, all groups). (A). A panel of genes corresponding to significant (P≤0.01) changes in gene expression in the comparison between young and old control groups was examined in both CR (left) and resveratrol fed mice (right). Numbers on X and Y axes represent fold changes in the young vs. old comparison (X axis), and the old CR vs old control (Y axis, left graphs) or old resveratrol vs. old control group (Y axis, right graphs). Each dot corresponds to an specific gene. Red dots correspond to genes that are significantly altered in expression at P≤0.01 in both aging and CR, or aging and resveratrol comparisons. Opposite fold changes in this analysis represent prevention of aging changes. (B). A panel of genes significantly changed by CR and resveratrol (but not by age) as compared to old control mice (P≤0.01) is plotted. Similar fold changes in this analysis represent resveratrol mimicry of CR. Pie charts represent the proportion of genes changed by CR only, resveratrol only or both treatments. Gene expression changes plotted in graphs correspond to the genes changed significantly expression by both treatments (red).