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Highly Specific Gene Silencing by Artificial miRNAs in Rice

Figure 1

Secondary Structure of the osa-MIR528 stemloop in pNW55.

Depicted is the secondary structure of the 255 bp derived from osa-MIR528 (245 bp plus the BamHI and KpNI restriction sites) as predicted by ‘RNAfold’ [33] (Vienna RNA package, http://www.tbi.univie.ac.at/RNA) for 23°C. The sequences replaced in the different transgenes are boxed. The oligo designer in WMD2 [25] (http://wmd2.weigelworld.org) designs the amRNA* sequence (black) such that it pairs to the respective miRNA (red) in the same way as in osa-MIR528.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0001829.g001