Calcitriol attenuates diethylnitrosamine-induced hepatic fibrosis in rats by reducing oxidative stress and fibrogenic mediators
Fig 3
Effects of calcitriol on liver morphology, histological staining, and quantification of Sirius Red-positive area in DEN-induced liver fibrosis.
(A) Representative images of gross liver morphology, (B) H&E staining, (C) Sirius Red staining for collagen deposition, (D) quantification of Sirius Red-positive area (%). Arrowhead indicates fibrotic regions; star indicates collagen deposition. Images were shown at 4x magnification. DEN induced a rough liver surface and increased collagen deposition, while calcitriol improved gross morphology and reduced fibrosis. Data are presented as mean ± SD, n = 6/group. *p < 0.05 when compared with the CON group, #p < 0.05 when compared with the DEN group.