High-intensity irreversible electroporation targeting intracellular structures enhance tumor ablation in lung cancer models
Fig 2
Cell Viability and apoptosis under various IREs in A549 cells.
(1) Cell viability after various electric field intensity treatment with pulse counts of 10, 20, and 40 pulses fixed at 20μs. (2) Cell viability at a fixed electric field of 4000 V/cm and 20 pulses, with pulse durations of 10, 20, and 40 μs. (3) Comparison of cell viability between 1000 V/cm and 4000 V/cm. (4) Apoptosis across increasing electric field strengths at a fixed pulse duration of 10 μs and pulse number of 20. (5) Apoptosis at electric field strength of 4,000 V/cm and 20 pulses with varying pulse durations of 10, 20, and 40 μs. (6) Comparison of apoptosis between 1000 V/cm and 4000 V/cm. Data are represented as mean ± SEM (n = 3). Multiple-group comparisons were analyzed using one-way ANOVA followed by Tukey’s HSD post hoc test, with significance indicated as: ###p < 0.001. Two-group tests were analyzed using unpaired two-tailed t-tests, with significance indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.