DNA source and primer choice affect the reliability of metabarcoding for nematode community profiling in agricultural soils
Fig 7
Nematode indices using different primer sets and DNA sources.
Differences among extraction methods and primer sets were assessed using linear mixed-effects models with subplot as a random effect. MI = maturity index, EI = enrichment index, SI = structure index. NE = Nematodes DNA extraction, SE = Soil DNA extraction, NemFopt = NemFopt–18Sr2bRopt, NemF = NemF–18Sr2b, and NF1 = NF1–18Sr2b. P = Primer set, M = Method of extraction and their interactions. ANOVA results: ***P < 0.001, **P < 0.01, *P < 0.05, n. s. = not significant.