DNA source and primer choice affect the reliability of metabarcoding for nematode community profiling in agricultural soils
Fig 2
Venn diagrams showing the number of nematode taxa detected using different primers and DNA sources.
(A) Identification at the genus level, and (B) Identification at the species level. NE = Nematodes DNA extraction, SE = Soil DNA extraction, NemFopt = NemFopt–18Sr2bRopt, NemF = NemF–18Sr2b, and NF1 = NF1–18Sr2b. SE.10 = Soil DNA extraction using 10 g of dry soil and SE.1.25 = Soil DNA extraction using 1.25 g of dry soil.