Longitudinal assessment of fluorescence stability shows fluorescence intensity decreases over time: implications for fluorescence microscopy studies
Fig 7
Fluorescence intensity changes over time in OB dopaminergic neurons.
(a-c) Chicken anti-TH primary antibody and AF488-conjugated secondary antibody-stained OB section imaged with widefield microscopy. (d) OB sections stained with chicken anti-TH primary antibody and AF488-conjugated secondary antibody show a significant decrease in fluorescence intensity. (e-g) Rabbit anti-TH primary antibody and AF488-conjugated secondary antibody-stained OB section imaged with widefield microscopy. (h) OB sections stained with rabbit anti-TH primary antibody and AF488-conjugated secondary antibody show a trend towards a decrease in fluorescence intensity that does not reach significance. (i-k) GCaMP6s fluorescence in OB dopaminergic neurons over time imaged with widefield microscopy. (l) OB sections imaged weekly for GCaMP6s fluorescence show significant decrease in fluorescence intensity. Images of weeks 1, 4 and 7 are shown as examples. Each line of data represents a single mouse.