Optimization and evaluation of a live virus SARS-CoV-2 neutralization assay
Fig 5
Sample matrix influence on specificity.
(A) Neutralization activity on 8 negative pre-pandemic serum samples, and (B) 8 negative EDTA plasma samples run in duplicates and run undiluted, 1:2.5, 1:5 and 1:10. (C) Neutralization activity on serum, EDTA or sodium heparin (NaHep) samples collected from two donors on the same day tested in duplicate and run undiluted, 1:2.5, 1:5 and 1:10. Virus: early pandemic strain (lineage B.1). Error bars represent standard deviations of duplicate determinations. ELISA primary antibody: rabbit mAb 40143-R019.