A strategic expression method of miR-29b and its anti-fibrotic effect based on RNA-sequencing analysis
Fig 2
Comparison of col1a1 and CMV promoter’s regulation of downstream gene expression.
(A) Both col1a1-luc and CMV-luc vectors were transfected into MEF cells for 24h. Luciferase activity was measured using a firefly luciferase substrate from Promega. (B) MEF cells transfected with col1a1-luc vector for 24 h were treated with TGF-β1 for an additional 24h and luciferase activity was measured. Each treatment was repeated 3 times (N = 3).