Expression and function of mechanosensitive ion channels in human valve interstitial cells
Fig 5
Role of TREK-1, TRPV4 and TRPC6/3 channels in stretch-induced changes in collagen mRNA expression in VICFB.
Changes in mRNA expression (relative to 18S) for COL I and COL III in VICFB under static conditions (white bars), after cyclic stretch (grey bars), and after stretch in the presence of either the TREK-1 channel inhibition by spadin, the general MSCNS blocker streptomycin, the TRPV4 inhibitor RN-9893 or the TRPC6/3 inhibitor GSK417651A (hatched bars) after 24 h of stretch. Gene expression was measured by RT-PCR and normalized to the housekeeping gene (n = 4 and 6 patients for COL I and III respectively; * = p<0.05, ** = p<0.01, *** = p<0.001).