All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
Fig 5
Pvalb7 expression pattern from early embryonic to late larval stages.
Whole mount in situ hybridization was performed in embryos and larvae at different developmental stages (1 dpf: A—C; 4 dpf: D—F; 10 dpf: G—I; 24 dpf J—L, enlarged in J´- L´) and illustrated in lateral (A, D, G, J, J´), dorsal (B, E, H, K, K´) and frontal views (C, F, I, L, L´). Brass wildtype line embryos (A—I) and casper line larvae (J–L, J´- L´) have been used. For staining, all embryos and larvae were incubated in BM purple for 22 hours.