Small molecule disruption of G protein βγ subunit signaling reprograms human macrophage phenotype and prevents autoimmune myocarditis in rats
Fig 5
Small molecule Gβγ-GRK2 inhibitor gallein suppressed M1-like macrophage phenotype marker expression in the hearts of EAM rats.
Western blots show specific bands for the expression of (A-E) IFNγ, COX2, HMGB1, TLR4 (ratio with GAPDH) and ERK1/2 (ratio with total ERK1/2). Each bar represents mean ± SEM, n = 3–4. Normal, age matched normal rats; EAM, rats with experimental autoimmune myocarditis treated with vehicle; EAM+G10, rats with EAM treated with gallein. ***p<0.001, **p<0.01 and *p<0.05 vs Normal; #p<0.05 vs EAM.