Dysregulation of NF-kB in glandular epithelial cells results in Sjögren’s-like features
Fig 2
Activated epithelial cells cause leukocyte striated duct invasion and local increased cytokine production.
A) Leukocytic (CD45+) invasion of a striated duct in A20-/- mice at 30 weeks of age. B) CD3+ T cell invasion of a striated duct in serial section to A. C) B220+ B cell invasion of a striated duct in serial section to A. Second panels in A-C are high resolution images of inset boxes. D) Quantification of invaded striated ducts frequency per 4 mm2 of glandular tissue. *** = p< 0.001, Two Way ANOVA. E) Relative expression of the pro-inflammatory cytokines (IFNα, IFNɣ, TNFα and IL-6) and pSS-associated chemokines (CXCL10, CXCL13) in A20-/- mice at 20 and 30 weeks of age. n ≥ 5 per group. * p < 0.05, ** p < 0.01, *** p < 0.001, student’s t-test.