Gene expression allelic imbalance in ovine brown adipose tissue impacts energy homeostasis
Fig 5
Enriched gene ontology terms for genes showing AI.
A. Functional enrichments for gene ontology (GO) terms associated with the down-sampled ranked list of genes showing AI. Only the top ranked 20 terms are shown. Functional enrichments were performed using a Wicoxon rank sum test implemented using GOseq [28]. q values less than 0.05 were considered significant. The GO term categories included Biological Process (black), Cell Component (blue) and Molecular Function (green). B. The top ranked 1,500 genes with AI after the down-sampling analysis were filtered to only include genes that showed significant AI (q<0.05) in the primary analysis. These 1,293 genes were examined for GO term functional enrichments using GOrilla [29] with a background gene list of all of the down-sampled ranked genes(q< 0.05). There were no term enrichments for the Molecular Function category. In both panels the q value for enrichment was -log10 transformed. Both analyses used a minimum read count threshold of 10.