Limb Ischemic Preconditioning Protects Endothelium from Oxidative Stress by Enhancing Nrf2 Translocation and Upregulating Expression of Antioxidases
Fig 5
Rat LIPC sera enhanced Nrf2 localization into nucleus inH2O2-injured HUVECs.
Localization of Nrf2 was performed by immunofluorescence and confocal microscopy. Nrf2 was stained with an anti-Nrf2 antibody and visualized with a secondary antibody conjugated with FITC (green). The nuclei were counterstained with DAPI (4’,-diamidino-2-phenylindole) staining indicating the location of the nucleus (blue). The merged image showed the nuclear location of Nrf2 protein.