Role of Siglec-7 in Apoptosis in Human Platelets
Figure 8
Ganglioside-induced platelet apoptosis is regulated by platelet P2Y1 and GPIIbIIIa antagonists (n = 10).
Platelets were pre-treated with antagonists for the corresponding platelet receptors: (A) MRS2179 100 µM: P2Y1 antagonist; (B) Tirofiban 10 µM: GPIIbIIIa antagonist; (C). Clopidogrel 476 µM: P2Y12 antagonist; (D) SCH79797 10 µM: PAR-1 antagonist; (E) tcY-NH2 400 µM: PAR-4 antagonist) or DMSO at the same dilution (negative control) followed by stimulation with GD2. The cell death rate (percentage of diminution in platelet counts) and ΔΨm depolarization (percentage of diminution in DIOC6(3) MFI) were prevented by P2Y1 and GPIIbIIIa antagonists. * Significant difference (t-test, p<0.05) between antagonist vs DMSO pretreated platelets.