cAMP Signaling Prevents Podocyte Apoptosis via Activation of Protein Kinase A and Mitochondrial Fusion
Figure 5
PKA signaling promoted mitochondria fusion in podocytes.
A and B: Western blot of podocytes treated with pCPT-cAMP for the indicated time. Bar graph of data obtained from five independent experiments. C and D: Western blot results from podocytes treated with pCPT-cAMP for 30 and 60 minutes. Bar graph of data obtained from at least five independent experiments. E: Western blot performed for podocytes treated with pCPT-cAMP for the indicated time. F and G: Western blot performed for podocytes treated with PAN or ADR in the presence or absence of pCPT-cAMP. H: Bar graph of data from five independent experiments. I: Western blot performed for podocytes treated with PAN or ADR in the presence or absence of pCPT-cAMP. J: Immunofluorescence and immunohistochemical staining of kidney from the control, ADR and forskolin+ADR groups (×200). *: P<0.05 compared with control group, #: P<0.05 compared with PAN group. pCPT: pCPT-cAMP.