A Pre- and Co-Knockdown of RNAseT Enzyme, Eri-1, Enhances the Efficiency of RNAi Induced Gene Silencing in Caenorhabditis elegans
Figure 4
Comparison of newly identified RNAi methodology performed in RNAi induced gene silencing of unc-73 in wild type background (A), RNAi induced gene silencing of unc-73 in rrf-3 background (B),Pre-incubation with eri-1 RNAi clone followed by co-incubation with unc-73 and eri-1 RNAi clones in wild type background (C), RNAi induced gene silencing of dpy-9 in wild type background (D), RNAi induced gene silencing of dpy-9 in rrf-3 background (E),Pre-incubation with eri-1 RNAi clone followed by co-incubation with dpy-9 and eri-1 RNAi clones in wild type background (F), RNAi induced gene silencing of bli-3 in wild type background (G), RNAi induced gene silencing of bli-3 in rrf-3 background (H),Pre-incubation with eri-1 RNAi clone followed by co-incubation with bli-3 and eri-1 RNAi clones in wild type background (I).
Fig 4J is a graphical presentation of worms showing percentages of unc/dpy/bli phenotypes examined by different RNAi methodologies. *p<0.05,**p<0.01, NS - Not significant.