Hematopoietic Protein-1 Regulates the Actin Membrane Skeleton and Membrane Stability in Murine Erythrocytes
Figure 6
Lifespan of transfused Hem1−/− erythrocytes is decreased compared to transfused WT erythrocytes.
Purified WT (open diamonds, n = 4 mice) and Hem1−/− (filled triangles, n = 3 mice) erythrocytes (5×108 per mouse) were labeled with CFSE dye and transfused via retro-orbital injection into Rag2−/−γc−/− host mice. Host mice were tail bled starting Day 1 post-transfusion and samples were analyzed via flow cytometry to determine the percentage of CFSE-labeled erythrocytes. Percent CFSE labeled cells remaining post transfusion were determined relative to Day 1 (100% labeling). P-values are noted above each time-point.