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Pearl Microstructure and Expression of Shell Matrix Protein Genes MSI31 and MSI60 in the Pearl Sac Epithelium of Pinctada fucata by In Situ Hybridization

Figure 5

Expression of MSI31 and MSI60 in pearl sac epithelium detected by ISH.

This oyster produced the pearl with pigmentation and a flaw (protrusion) shown in Fig. 4. Images in the upper and lower rows show the expression of MSI60 and MSI31, respectively. The numbers refer to sequence of serial sections. Sections 1 and 30 corresponded to the plane indicated by arrows on Fig. 4A (sections 27–30 are not shown). Scale bar is100 µm.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0052372.g005