Large-Scale In Vitro Expansion of Polyclonal Human Switched-Memory B Lymphocytes
Figure 5
Validation of expansion during long-term culture.
Three switched-memory B lymphocyte samples were cultured as described in Fig. 1 and transferred in petri dishes to test the feasibility of increasing the culture volume up to 500 mL. (A) Expansion factors were similar to those obtained in 6-well plates (Fig. 1). (B) Culture volumes are shown as a function of time. (C) IgA, IgG and IgM concentrations were determined in supernatants of the three independent samples at the end of the culture. (D) Flow cytometry analyses for kappa and lambda chain expression was similar for all three independent samples.