Detection of Prion Protein Particles in Blood Plasma of Scrapie Infected Sheep
Figure 5
Optimized image processing and evaluation.
(A) Image raw data of control sample no. 6 (top) and a scrapie-positive sample no. 7 (bottom). Depicted is one representative out of nine images taken for each sample. Scale bar = 50 µm. (B) Optimization of ‘rolling ball’ radius. After applying rolling ball background subtraction an intensity cutoff (500) was applied. Smaller radii of 1 and 2 pixels (arrows) allow for differentiation of the positive and the control sample (negative). (C) Optimized background removal parameters were applied to the complete panel of samples.