Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
Figure 2
Vaccine Comparisons of Three SARS-CoV Vaccines, Experiment 1.
Mean lung cellular infiltration/lesion pathology and percent eosinophils in infiltrates for each vaccine dosage group two days after challenge with SARS-CoV. A. Mean lesion score and standard error of the mean (S.E.) for each vaccine dosage group. All mice exhibited lung histopathology. Scores are mean of scores for seven to eight mice per group. Scoring. 0 – no pathology, 1 and 2 – (1) minimal (2) moderate peribronchiole and perivascular cellular infiltration, 3 and 4 – 1 and/or 2 plus minimal (3) or moderate (4) epithelial cell necrosis of bronchioles with cell debris in the lumen. B. Mean percent eosinophils on histologic evaluation for seven to eight mice in each vaccine dosage group. Mean for each mouse is the mean percent eosinophils on five separate microscopy fields of lung sections. Analyses: A. Mean lesion scores were different p<.001. DIV without alum greater than with alum p = .001, VLP without alum greater than with alum p = .008. Posthoc comparisons: DIV lower than SV p = .001 and controls p<.001 but not VLP p>.05. SV lower than controls p .048. B. Mean percent eosinophils were different p<.001. Mean percent eosinophils lower for DIV with alum than without alum p = .049 and lower for SV with alum than without alum p = .001. Mean percent eosinophils lower for SV than DIV p = .002 or VLP. P = <.001. Mean percent eosinophils greater than controls for DIV, SV and VLP, all three vaccines p<.001.