Stanniocalcin-1 Regulates Extracellular ATP-Induced Calcium Waves in Human Epithelial Cancer Cells by Stimulating ATP Release from Bystander Cells
Figure 7
STC1 Enhanced the Release of ATP in vitro and in vivo.
A) A549 cells were stimulated with 500 ng/mL STC1 for 10 mins. Conditioned medium was collected and assayed for ATP. Adherent cells were lysed and measured for ATP and DNA content. ATP values were normalized to DNA fluorescence. * = p<0.05; n = 3. B) A549 cells were stimulated with 10 µM ATP for 2, 5 and 10 mins. Assay of conditioned medium and cell lysates of wild type and STC1 over-expressing MEFs. * = p<0.05; n = 3. C) Serum was isolated from wild type and STC1 transgenic mice and assayed for ATP content. * = p<0.05; n>17.