Stanniocalcin-1 Regulates Extracellular ATP-Induced Calcium Waves in Human Epithelial Cancer Cells by Stimulating ATP Release from Bystander Cells
Figure 6
STC1 and Extracellular ATP Were Required for Calcium Wave Propagation following Mechanical Stimulation.
A) Fluo-4 labeled A549 monolayers were mechanically stimulated in the presence of isotype antibody (control), an STC1 blocking antibody (anti-STC1; 1 µg/mL) or apyrase (250 mU/mL) and assayed by live cell microscopy. B) Distance of calcium wave propagation from A. Error bars = SD. * = p<0.05. C) Quantification of signal intensity adjacent to scrape site following mechanical stimulation. Error bars = SD. * = p<0.05.