Detection of pathogenic Leptospira with rapid extraction followed by recombinase polymerase amplification (RPA) and quantitative polymerase chain reaction (qPCR) assay-A comprehensive study from Sri Lanka

• Hansi Uduwawala ,

  Contributed equally to this work with: Hansi Uduwawala, Gayana P. S. Gunaratna

  Roles Formal analysis, Investigation, Methodology, Validation, Writing – original draft

  Affiliation Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

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• Aresha Manamperi ,

  Roles Conceptualization, Funding acquisition, Methodology, Project administration, Supervision, Writing – review & editing

  * E-mail: areshamanamperi@kln.ac.lk (AM); ahmed.abd_el_wahed@uni-leipzig.de (AAEW)

  ¶‡ AM, AAEW and MH also contributed equally to this work.

  Affiliation Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

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• Gayana P. S. Gunaratna ,

  Contributed equally to this work with: Hansi Uduwawala, Gayana P. S. Gunaratna

  Roles Formal analysis, Methodology, Writing – review & editing

  Affiliation Department of Medical Microbiology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

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• Lilani Karunanayake,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation National Reference Laboratory for Leptospirosis, Medical Research Institute, Colombo, Sri Lanka

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• Arianna Ceruti,

  Roles Methodology, Writing – review & editing

  Affiliation Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany

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• Ahmed Abd El Wahed ,

  Roles Conceptualization, Funding acquisition, Methodology, Resources, Supervision, Validation, Writing – review & editing

  * E-mail: areshamanamperi@kln.ac.lk (AM); ahmed.abd_el_wahed@uni-leipzig.de (AAEW)

  ¶‡ AM, AAEW and MH also contributed equally to this work.

  Affiliation Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany

  https://orcid.org/0000-0003-3347-6075

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• Lakkumar Fernando,

  Roles Investigation, Writing – review & editing

  Affiliation Centre for Clinical Management of Dengue & Dengue Haemorrhagic Fever, District General Hospital, Negombo, Sri Lanka

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• Ranjan Premaratna,

  Roles Investigation, Writing – review & editing

  Affiliation Department of Medicine, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

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• Menaka Hapugoda

  Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  ¶‡ AM, AAEW and MH also contributed equally to this work.

  Affiliation Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

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