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Open Access
Peer-reviewed
Lab Protocol
Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format
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Yan Chen,
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing
Affiliations Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, DOE Joint BioEnergy Institute, Emeryville, California, United States of America, DOE Agile BioFoundry, Emeryville, California, United States of America
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Jennifer W. Gin,
Roles Data curation, Investigation, Methodology, Writing – review & editing
Affiliations Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, DOE Joint BioEnergy Institute, Emeryville, California, United States of America, DOE Agile BioFoundry, Emeryville, California, United States of America
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Ying Wang,
Roles Investigation, Writing – review & editing
Affiliation Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
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Markus de Raad,
Roles Investigation, Writing – review & editing
Affiliation Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
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Stephen Tan,
Roles Investigation, Writing – review & editing
Affiliations Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, DOE Joint BioEnergy Institute, Emeryville, California, United States of America, DOE Agile BioFoundry, Emeryville, California, United States of America
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Nathan J. Hillson,
Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing
Affiliations Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, DOE Joint BioEnergy Institute, Emeryville, California, United States of America, DOE Agile BioFoundry, Emeryville, California, United States of America
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Trent R. Northen,
Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing
Affiliations DOE Joint BioEnergy Institute, Emeryville, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
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Paul D. Adams,
Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing
Affiliations DOE Joint BioEnergy Institute, Emeryville, California, United States of America, Department of Bioengineering, University of California Berkeley, Berkeley, California, United States of America, Molecular Biophysics and Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
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Christopher J. Petzold
Roles Conceptualization, Formal analysis, Supervision, Visualization, Writing – original draft, Writing – review & editing
* E-mail: cjpetzold@lbl.gov
Affiliations Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, DOE Joint BioEnergy Institute, Emeryville, California, United States of America, DOE Agile BioFoundry, Emeryville, California, United States of America
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Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format
- Yan Chen,
- Jennifer W. Gin,
- Ying Wang,
- Markus de Raad,
- Stephen Tan,
- Nathan J. Hillson,
- Trent R. Northen,
- Paul D. Adams,
- Christopher J. Petzold
- Published: July 7, 2023
- https://doi.org/10.1371/journal.pone.0288102