Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection

• Yukiko Nakura,

  Roles Data curation, Investigation, Visualization, Writing – original draft

  Affiliation Department of Developmental Medicine, Research Institute, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

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• Heng Ning Wu,

  Roles Investigation

  Affiliation Department of Developmental Medicine, Research Institute, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

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• Yuya Okamoto,

  Roles Resources

  Affiliation Department of Laboratory Medicine, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

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• Muneyuki Takeuchi,

  Roles Resources

  Affiliation Department of Intensive Care Medicine, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

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• Koichiro Suzuki,

  Roles Validation

  Affiliation The Research Foundation for Microbial Diseases of Osaka University, Suita-city, Osaka, Japan

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• Yoshitaka Tamura,

  Roles Resources

  Affiliation Department of Clinical Laboratory, Osaka Habikino Medical Center, Habikino-city, Osaka, Japan

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• Yuichiro Oba,

  Roles Resources

  Affiliation Department of General Medicine, Osaka General Medical Center, Osaka-city, Osaka, Japan

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• Fumiko Nishiumi,

  Roles Investigation

  Affiliation Department of Developmental Medicine, Research Institute, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

  https://orcid.org/0000-0003-0383-1179

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• Nobuaki Hatori,

  Roles Validation

  Affiliation The Research Foundation for Microbial Diseases of Osaka University, Suita-city, Osaka, Japan

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• Shinsuke Fujiwara,

  Roles Funding acquisition, Resources, Writing – review & editing

  Affiliation Department of Biosciences, School of Biological and Environmental Sciences, Kwansei-Gakuin University, Sanda-city, Hyogo, Japan

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• Kiyoshi Yasukawa,

  Roles Funding acquisition, Resources, Writing – review & editing

  Affiliation Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto-city, Kyoto, Japan

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• Shinobu Ida,

  Roles Resources

  Affiliation Department of Laboratory Medicine, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

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• Itaru Yanagihara

  Roles Conceptualization, Funding acquisition, Visualization, Writing – original draft

  * E-mail: itaruy@wch.opho.jp

  Affiliations Department of Developmental Medicine, Research Institute, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan, Department of Laboratory Medicine, Osaka Women’s and Children’s Hospital, Izumi-city, Osaka, Japan

  https://orcid.org/0000-0001-9882-8196

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