A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus

• Daniel Conde ,

  Contributed equally to this work with: Daniel Conde, Paolo M. Triozzi

  Roles Conceptualization, Methodology, Writing – original draft, Writing – review & editing

  Affiliation School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America

  https://orcid.org/0000-0001-8362-4190

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• Paolo M. Triozzi ,

  Contributed equally to this work with: Daniel Conde, Paolo M. Triozzi

  Roles Conceptualization, Methodology, Writing – original draft, Writing – review & editing

  Affiliation School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America

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• Kelly M. Balmant,

  Roles Data curation, Writing – original draft, Writing – review & editing

  Affiliation School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America

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• Andria L. Doty,

  Roles Methodology, Writing – original draft

  Affiliation Interdisciplinary Center for Biotechnology, Flow Cytometry & Imaging Core, University of Florida, Gainesville, Florida, United States of America

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• Mariza Miranda,

  Roles Methodology

  Affiliation Interdisciplinary Center for Biotechnology, Flow Cytometry & Imaging Core, University of Florida, Gainesville, Florida, United States of America

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• Anthony Boullosa,

  Roles Methodology

  Affiliation Interdisciplinary Center for Biotechnology, Flow Cytometry & Imaging Core, University of Florida, Gainesville, Florida, United States of America

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• Henry W. Schmidt,

  Roles Conceptualization, Writing – review & editing

  Affiliation School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America

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• Wendell J. Pereira,

  Roles Supervision, Writing – review & editing

  Affiliation School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America

  https://orcid.org/0000-0003-1019-6281

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• Christopher Dervinis,

  Roles Resources, Supervision, Writing – review & editing

  Affiliation School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America

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• Matias Kirst

  Roles Conceptualization, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: mkirst@ufl.edu

  Affiliations School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America, Genetics Institute, University of Florida, Gainesville, Florida, United States of America

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