Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Martine Bangratz; Issa Wonni; Kossi Kini; Moussa Sondo; Christophe Brugidou; Gilles Béna; Fatoumata Gnacko; Mariam Barro; Ralf Koebnik; Drissa Silué; Charlotte Tollenaere

doi:10.1371/journal.pone.0232115

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Open Access

Peer-reviewed

Research Article

Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Martine Bangratz,

Roles Conceptualization, Data curation, Investigation, Methodology, Supervision, Writing – review & editing

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso
⨯
Issa Wonni,

Roles Conceptualization, Methodology, Project administration, Supervision

Affiliation INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso
⨯
Kossi Kini,

Roles Conceptualization, Investigation, Methodology, Writing – review & editing

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, AfricaRice, Plant Pathology, Bouaké, Ivory Coast

http://orcid.org/0000-0001-9917-477X

⨯
Moussa Sondo,

Roles Data curation, Investigation

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso
⨯
Christophe Brugidou,

Roles Funding acquisition, Project administration, Writing – review & editing

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso
⨯
Gilles Béna,

Roles Investigation, Methodology, Writing – review & editing

Affiliation IRD, Cirad, Univ Montpellier, IPME, Montpellier, France
⨯
Fatoumata Gnacko,

Roles Investigation

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso
⨯
Mariam Barro,

Roles Data curation, Investigation

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso
⨯
Ralf Koebnik,

Roles Conceptualization, Writing – review & editing

Affiliation IRD, Cirad, Univ Montpellier, IPME, Montpellier, France
⨯
Drissa Silué,

Roles Conceptualization, Methodology, Supervision, Writing – review & editing

Affiliation AfricaRice, Plant Pathology, Bouaké, Ivory Coast

http://orcid.org/0000-0003-1922-7179

⨯
Charlotte Tollenaere

Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing – original draft

* E-mail: charlotte.tollenaere@ird.fr

Affiliations IRD, Cirad, Univ Montpellier, IPME, Montpellier, France, INERA, Laboratoire de Phytopathologie, LMI PathoBios, Bobo-Dioulasso, Burkina Faso

http://orcid.org/0000-0003-2045-2824

⨯

Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Martine Bangratz,
Issa Wonni,
Kossi Kini,
Moussa Sondo,
Christophe Brugidou,
Gilles Béna,
Fatoumata Gnacko,
Mariam Barro,
Ralf Koebnik,
Drissa Silué

Published: April 27, 2020
https://doi.org/10.1371/journal.pone.0232115

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