Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop

• Philippe Champeil ,

  Contributed equally to this work with: Philippe Champeil, Béatrice de Foresta

  Roles Investigation, Methodology, Project administration, Supervision, Writing – original draft

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

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• Béatrice de Foresta ,

  Contributed equally to this work with: Philippe Champeil, Béatrice de Foresta

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

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• Martin Picard,

  Roles Investigation, Methodology, Writing – review & editing

  Current address: Université de Paris, CNRS UMR 7099 Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS, Paris, France.; Institut de Biologie Physico-Chimique, Fondation Edmond de Rothschild pour le développement de la recherche Scientifique, Paris, France.

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

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• Carole Gauron,

  Roles Investigation, Methodology, Writing – review & editing

  Current address: IRD, UMR-IPME, EAVIR, Montpellier, France.

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

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• Dominique Georgin,

  Roles Resources, Writing – review & editing

  Affiliation CEA, DSV, Institut des Sciences du Vivant Frédéric Joliot, Service de Chimie Biorganique et de Marquage, Gif-sur-Yvette, France

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• Marc le Maire,

  Roles Writing – review & editing

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

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• Jesper V. Møller,

  Roles Writing – review & editing

  Affiliations Center for Membrane Pumps in Cells and Disease, PUMPKIN, Danish National Research Foundation, Aarhus University, Aarhus, Denmark, Department of Biomedicine, Aarhus University, Aarhus, Denmark

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• Guillaume Lenoir,

  Roles Writing – review & editing

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

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• Cédric Montigny

  Roles Investigation, Methodology, Writing – review & editing

  * E-mail: cedric.montigny@i2bc.paris-saclay.fr

  Affiliation Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

  http://orcid.org/0000-0003-0905-9861

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