Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy

Joel Pel; Wendy W. Y. Choi; Amy Leung; Gosuke Shibahara; Laura Gelinas; Milenko Despotovic; W. Lloyd Ung; Andre Marziali

doi:10.1371/journal.pone.0204265

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Open Access

Peer-reviewed

Research Article

Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy

Joel Pel,

Roles Conceptualization, Data curation, Formal analysis, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada

http://orcid.org/0000-0003-0272-0477

⨯
Wendy W. Y. Choi,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada
⨯
Amy Leung,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada
⨯
Gosuke Shibahara,

Roles Conceptualization, Data curation, Formal analysis, Software, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada

http://orcid.org/0000-0001-8871-0952

⨯
Laura Gelinas,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada
⨯
Milenko Despotovic,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Software, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada
⨯
W. Lloyd Ung,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Software, Writing – review & editing

Affiliation Boreal Genomics Inc., Vancouver, British Columbia, Canada
⨯
Andre Marziali

Roles Conceptualization, Writing – original draft, Writing – review & editing

* E-mail: andre@phas.ubc.ca

Affiliations Boreal Genomics Inc., Vancouver, British Columbia, Canada, Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia, Canada
⨯

Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy

Joel Pel,
Wendy W. Y. Choi,
Amy Leung,
Gosuke Shibahara,
Laura Gelinas,
Milenko Despotovic,
W. Lloyd Ung,
Andre Marziali

Published: October 2, 2018
https://doi.org/10.1371/journal.pone.0204265
>> See the preprint

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Sequence alignment
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