Method Specific Calibration Corrects for DNA Extraction Method Effects on Relative Telomere Length Measurements by Quantitative PCR

Luise A. Seeker; Rebecca Holland; Sarah Underwood; Jennifer Fairlie; Androniki Psifidi; Joanna J. Ilska; Ainsley Bagnall; Bruce Whitelaw; Mike Coffey; Georgios Banos; Daniel H. Nussey

doi:10.1371/journal.pone.0164046

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Open Access

Peer-reviewed

Research Article

Method Specific Calibration Corrects for DNA Extraction Method Effects on Relative Telomere Length Measurements by Quantitative PCR

Luise A. Seeker ,

* E-mail: Luise.seeker@sruc.ac.uk

Affiliations Animal & Veterinary Sciences, SRUC, Roslin Institute Building, Easter Bush, Midlothian EH25 9RG, United Kingdom, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United Kingdom

http://orcid.org/0000-0001-6375-6372

⨯
Rebecca Holland,

Affiliation Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Midlothian, United Kingdom
⨯
Sarah Underwood,

Affiliation Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Midlothian, United Kingdom
⨯
Jennifer Fairlie,

Affiliation Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Midlothian, United Kingdom
⨯
Androniki Psifidi,

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United Kingdom
⨯
Joanna J. Ilska,

Affiliation Animal & Veterinary Sciences, SRUC, Roslin Institute Building, Easter Bush, Midlothian EH25 9RG, United Kingdom
⨯
Ainsley Bagnall,

Affiliation SRUC Crichton Royal Farm, Glencaple Road, Dumfries, United Kingdom
⨯
Bruce Whitelaw,

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United Kingdom
⨯
Mike Coffey,

Affiliation Animal & Veterinary Sciences, SRUC, Roslin Institute Building, Easter Bush, Midlothian EH25 9RG, United Kingdom
⨯
Georgios Banos,

Affiliations Animal & Veterinary Sciences, SRUC, Roslin Institute Building, Easter Bush, Midlothian EH25 9RG, United Kingdom, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United Kingdom
⨯
Daniel H. Nussey

Affiliation Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Midlothian, United Kingdom
⨯

Method Specific Calibration Corrects for DNA Extraction Method Effects on Relative Telomere Length Measurements by Quantitative PCR

Luise A. Seeker,
Rebecca Holland,
Sarah Underwood,
Jennifer Fairlie,
Androniki Psifidi,
Joanna J. Ilska,
Ainsley Bagnall,
Bruce Whitelaw,
Mike Coffey,
Georgios Banos

Published: October 10, 2016
https://doi.org/10.1371/journal.pone.0164046

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DNA extraction
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Cattle
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Telomeres
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Telomere length
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Quality control
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Sheep
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Measurement
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