A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

Peter J. Romanienko; Joseph Giacalone; Joanne Ingenito; Yijie Wang; Mayumi Isaka; Thomas Johnson; Yun You; Willie H. Mark

doi:10.1371/journal.pone.0148362

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Peer-reviewed

Research Article

A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

Peter J. Romanienko ,

* E-mail: romaniep@mskcc.org

Affiliations Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America, Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
⨯
Joseph Giacalone,

Affiliation Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
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Joanne Ingenito,

Affiliation Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
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Yijie Wang,

Affiliation Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
⨯
Mayumi Isaka,

Affiliation Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
⨯
Thomas Johnson,

Affiliation Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
⨯
Yun You,

Affiliations Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America, Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
⨯
Willie H. Mark

Affiliations Mouse Genetics Core Facility, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America, Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, United States of America
⨯

A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

Peter J. Romanienko,
Joseph Giacalone,
Joanne Ingenito,
Yijie Wang,
Mayumi Isaka,
Thomas Johnson,
Yun You,
Willie H. Mark

Published: February 5, 2016
https://doi.org/10.1371/journal.pone.0148362

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24 Aug 2016
14:16 GMT
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New contact info for corresponding author Posted by promanienko on 24 Aug 2016 at 14:16 GMT

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