Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

Open Access

Peer-reviewed

Research Article

Moushree Pal Roy,

Affiliation Department of Biotechnology, University of North Bengal, Siliguri, India
⨯
Deepika Mazumdar,

Affiliation Department of Biotechnology, University of North Bengal, Siliguri, India
⨯
Subhabrata Dutta,

Affiliation Department of Biotechnology, University of North Bengal, Siliguri, India
⨯
Shyama Prasad Saha,

Affiliation Department of Biotechnology, University of North Bengal, Siliguri, India
⨯
Shilpi Ghosh

* E-mail: ghosshilpi@gmail.com

Affiliation Department of Biotechnology, University of North Bengal, Siliguri, India
⨯

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Shigella
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Plasmid construction
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Recombinant proteins
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Transformation associated recombination
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Phylogenetic analysis
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Signal peptides
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Cloning
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Serine proteases
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