SYBR Green Real-Time PCR-RFLP Assay Targeting the Plasmodium Cytochrome B Gene – A Highly Sensitive Molecular Tool for Malaria Parasite Detection and Species Determination

Weiping Xu; Ulrika Morris; Berit Aydin-Schmidt; Mwinyi I. Msellem; Delér Shakely; Max Petzold; Anders Björkman; Andreas Mårtensson

doi:10.1371/journal.pone.0120210

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Peer-reviewed

Research Article

SYBR Green Real-Time PCR-RFLP Assay Targeting the Plasmodium Cytochrome B Gene – A Highly Sensitive Molecular Tool for Malaria Parasite Detection and Species Determination

Weiping Xu ,

* E-mail: Weiping.Xu@ki.se

Current address: School of Food and Environment, Dalian University of Technology, Panjin Campus, Liaoning, 124221, China

Affiliation Department of Microbiology, Tumor and Cell Biology, Division of Malaria Research, Karolinska Institutet, Stockholm, Sweden
⨯
Ulrika Morris,

Affiliation Department of Microbiology, Tumor and Cell Biology, Division of Malaria Research, Karolinska Institutet, Stockholm, Sweden
⨯
Berit Aydin-Schmidt,

Affiliation Department of Microbiology, Tumor and Cell Biology, Division of Malaria Research, Karolinska Institutet, Stockholm, Sweden
⨯
Mwinyi I. Msellem,

Affiliation Zanzibar Malaria Elimination Programme, Zanzibar, Tanzania
⨯
Delér Shakely,

Affiliation Department of Microbiology, Tumor and Cell Biology, Division of Malaria Research, Karolinska Institutet, Stockholm, Sweden
⨯
Max Petzold,

Affiliation Center for Applied Biostatistics, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
⨯
Anders Björkman,

Affiliation Department of Microbiology, Tumor and Cell Biology, Division of Malaria Research, Karolinska Institutet, Stockholm, Sweden
⨯
Andreas Mårtensson

Affiliations Department of Microbiology, Tumor and Cell Biology, Division of Malaria Research, Karolinska Institutet, Stockholm, Sweden, Department of Public Health Sciences, Division of Global Health (IHCAR), Karolinska Institutet, Stockholm, Sweden, Centre for Clinical Research Sörmland, Uppsala University, Uppsala, Sweden
⨯

SYBR Green Real-Time PCR-RFLP Assay Targeting the Plasmodium Cytochrome B Gene – A Highly Sensitive Molecular Tool for Malaria Parasite Detection and Species Determination

Weiping Xu,
Ulrika Morris,
Berit Aydin-Schmidt,
Mwinyi I. Msellem,
Delér Shakely,
Max Petzold,
Anders Björkman,
Andreas Mårtensson

Published: March 16, 2015
https://doi.org/10.1371/journal.pone.0120210

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Plasmodium
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Polymerase chain reaction
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Malarial parasites
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Restriction fragment length polymorphism analysis
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DNA extraction
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Ribosomal RNA
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