A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly

Open Access

Peer-reviewed

Research Article

Theodore L. Roth,

Affiliation Departments of Developmental Biology, Genetics, Bioengineering, & Biology, Stanford University School of Medicine, Stanford, California, United States of America
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Ljiljana Milenkovic,

Affiliation Departments of Developmental Biology, Genetics, Bioengineering, & Biology, Stanford University School of Medicine, Stanford, California, United States of America
⨯
Matthew P. Scott

* E-mail: mscott@stanford.edu

Affiliation Departments of Developmental Biology, Genetics, Bioengineering, & Biology, Stanford University School of Medicine, Stanford, California, United States of America
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DNA fragment ligation
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Polymerase chain reaction
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Ligases
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Oligonucleotides
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Molecular cloning
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Plasmid construction
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DNA annealing
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Denaturation
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