Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

• Bin Shen ,

  Contributed equally to this work with: Bin Shen, Xin Zhang

  Affiliation MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing, China

  ⨯
• Xin Zhang ,

  Contributed equally to this work with: Bin Shen, Xin Zhang

  Affiliation College of Life Sciences, Wuhan University, Wuhan, China

  ⨯
• Yinan Du,

  Affiliation MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing, China

  ⨯
• Jianying Wang,

  Affiliation MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing, China

  ⨯
• Jun Gong,

  Affiliation College of Life Sciences, Wuhan University, Wuhan, China

  ⨯
• Xiaodong Zhang,

  Affiliation College of Life Sciences, Wuhan University, Wuhan, China

  ⨯
• Peri H. Tate,

  Affiliation Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom

  ⨯
• Hongliang Li,

  Affiliation College of Life Sciences, Wuhan University, Wuhan, China

  ⨯
• Xingxu Huang ,

  * E-mail: xingxuhuang@mail.nju.edu.cn (WZ); wz1@sanger.ac.uk (XH)

  Affiliation MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing, China

  ⨯
• Wensheng Zhang

  * E-mail: xingxuhuang@mail.nju.edu.cn (WZ); wz1@sanger.ac.uk (XH)

  Affiliation Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom

  ⨯