Direct Quantification of mRNA and miRNA from Cell Lysates Using Reverse Transcription Real Time PCR: A Multidimensional Analysis of the Performance of Reagents and Workflows

Open Access

Peer-reviewed

Research Article

Yoon Khei Ho,

Affiliation Department of Biochemistry, National University of Singapore, Singapore, Singapore
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Wen Ting Xu,

Affiliation Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore
⨯
Heng Phon Too

* E-mail: bchtoohp@nus.edu.sg

Affiliations Department of Biochemistry, National University of Singapore, Singapore, Singapore, Molecular Engineering of Biological and Chemical System/Chemical Pharmaceutical Engineering, Singapore–Massachusetts Institute of Technology Alliance, Singapore, Singapore, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), Singapore, Singapore
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MicroRNAs
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Polymerase chain reaction
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Gene expression
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Messenger RNA
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Reverse transcription
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RNA isolation
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Microarrays
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Lysis (medicine)
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