Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis

Takeshi Hanami; Diane Delobel; Hajime Kanamori; Yuki Tanaka; Yasumasa Kimura; Ayako Nakasone; Takahiro Soma; Yoshihide Hayashizaki; Kengo Usui; Matthias Harbers

doi:10.1371/journal.pone.0070942

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Open Access

Peer-reviewed

Research Article

Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis

Takeshi Hanami ,

Contributed equally to this work with: Takeshi Hanami, Diane Delobel

Affiliation Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Diane Delobel ,

Contributed equally to this work with: Takeshi Hanami, Diane Delobel

Affiliation Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Hajime Kanamori,

Affiliation K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Yuki Tanaka,

Affiliation Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Yasumasa Kimura,

Affiliation Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Ayako Nakasone,

Affiliation K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Takahiro Soma,

Affiliation Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Yoshihide Hayashizaki,

Affiliation RIKEN Preventive Medicine and Diagnosis Innovation Program, Yokohama, Kanagawa, Japan
⨯
Kengo Usui ,

* E-mail: matthias.harbers@riken.jp (MH); kusui@gsc.riken.jp (KU)

Affiliations Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan, RIKEN Preventive Medicine and Diagnosis Innovation Program, Yokohama, Kanagawa, Japan
⨯
Matthias Harbers

* E-mail: matthias.harbers@riken.jp (MH); kusui@gsc.riken.jp (KU)

Affiliations Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan, K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯

Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis

Takeshi Hanami,
Diane Delobel,
Hajime Kanamori,
Yuki Tanaka,
Yasumasa Kimura,
Ayako Nakasone,
Takahiro Soma,
Yoshihide Hayashizaki,
Kengo Usui,
Matthias Harbers

Published: August 7, 2013
https://doi.org/10.1371/journal.pone.0070942

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Polymerase chain reaction
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Melting
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Probe hybridization
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Signal amplification
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Oligonucleotides
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Fluorescent dyes
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DNA polymerase
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