MHC Multimer-Guided and Cell Culture-Independent Isolation of Functional T Cell Receptors from Single Cells Facilitates TCR Identification for Immunotherapy

Georg Dössinger; Mario Bunse; Jeannette Bet; Julia Albrecht; Paulina J. Paszkiewicz; Bianca Weißbrich; Isabell Schiedewitz; Lynette Henkel; Matthias Schiemann; Michael Neuenhahn; Wolfgang Uckert; Dirk H. Busch

doi:10.1371/journal.pone.0061384

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Open Access

Peer-reviewed

Research Article

MHC Multimer-Guided and Cell Culture-Independent Isolation of Functional T Cell Receptors from Single Cells Facilitates TCR Identification for Immunotherapy

Georg Dössinger,

Affiliation Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany
⨯
Mario Bunse,

Affiliation Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
⨯
Jeannette Bet,

Affiliations Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany, Focus Group “Clinical Cell Processing and Purification”, Institute for Advanced Study, Technische Universität München, Munich, Germany
⨯
Julia Albrecht,

Affiliation Clinical Cooperation Groups ‘‘Antigen-specific Immunotherapy’’ and “Immune Monitoring”, Helmholtz Center Munich (Neuherberg) and Technische Universität München, Munich, Germany
⨯
Paulina J. Paszkiewicz,

Affiliations Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany, Focus Group “Clinical Cell Processing and Purification”, Institute for Advanced Study, Technische Universität München, Munich, Germany
⨯
Bianca Weißbrich,

Affiliations Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany, Focus Group “Clinical Cell Processing and Purification”, Institute for Advanced Study, Technische Universität München, Munich, Germany
⨯
Isabell Schiedewitz,

Affiliation Clinical Cooperation Groups ‘‘Antigen-specific Immunotherapy’’ and “Immune Monitoring”, Helmholtz Center Munich (Neuherberg) and Technische Universität München, Munich, Germany
⨯
Lynette Henkel,

Affiliation Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany
⨯
Matthias Schiemann,

Affiliations Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany, Clinical Cooperation Groups ‘‘Antigen-specific Immunotherapy’’ and “Immune Monitoring”, Helmholtz Center Munich (Neuherberg) and Technische Universität München, Munich, Germany
⨯
Michael Neuenhahn,

Affiliations Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany, Clinical Cooperation Groups ‘‘Antigen-specific Immunotherapy’’ and “Immune Monitoring”, Helmholtz Center Munich (Neuherberg) and Technische Universität München, Munich, Germany
⨯
Wolfgang Uckert,

Affiliations Max-Delbrück-Center for Molecular Medicine, Berlin, Germany, Humboldt-Universität, Berlin, Germany
⨯
Dirk H. Busch

* E-mail: dirk.busch@mikrobio.med.tum.de

Affiliations Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany, Focus Group “Clinical Cell Processing and Purification”, Institute for Advanced Study, Technische Universität München, Munich, Germany, Clinical Cooperation Groups ‘‘Antigen-specific Immunotherapy’’ and “Immune Monitoring”, Helmholtz Center Munich (Neuherberg) and Technische Universität München, Munich, Germany, DZIF - National Centre for Infection Research, Munich, Germany
⨯

MHC Multimer-Guided and Cell Culture-Independent Isolation of Functional T Cell Receptors from Single Cells Facilitates TCR Identification for Immunotherapy

Georg Dössinger,
Mario Bunse,
Jeannette Bet,
Julia Albrecht,
Paulina J. Paszkiewicz,
Bianca Weißbrich,
Isabell Schiedewitz,
Lynette Henkel,
Matthias Schiemann,
Michael Neuenhahn

Published: April 26, 2013
https://doi.org/10.1371/journal.pone.0061384

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