Characterization and Quantification of Intact 26S Proteasome Proteins by Real-Time Measurement of Intrinsic Fluorescence Prior to Top-down Mass Spectrometry

• Jason D. Russell,

  Affiliations Department of Chemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States of America, Genome Center of Wisconsin, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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• Mark Scalf,

  Affiliation Department of Chemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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• Adam J. Book,

  Affiliation Department of Genetics, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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• Daniel T. Ladror,

  Affiliation Department of Chemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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• Richard D. Vierstra,

  Affiliation Department of Genetics, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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• Lloyd M. Smith,

  Affiliations Department of Chemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States of America, Genome Center of Wisconsin, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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• Joshua J. Coon

  * E-mail: jcoon@chem.wisc.edu

  Affiliations Department of Chemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States of America, Department of Biomolecular Chemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States of America, Department of Genetics, University of Wisconsin – Madison, Madison, Wisconsin, United States of America, Genome Center of Wisconsin, University of Wisconsin – Madison, Madison, Wisconsin, United States of America

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