Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction for the Detection of Human Enterovirus 71 Subgenotype C4 in Nasopharyngeal Swab Specimens

Kai Nie; Shun-xiang Qi; Yong Zhang; Le Luo; Yun Xie; Meng-jie Yang; Yi Zhang; Jin Li; Hongwei Shen; Qi Li; Xue-jun Ma

doi:10.1371/journal.pone.0052486

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Open Access

Peer-reviewed

Research Article

Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction for the Detection of Human Enterovirus 71 Subgenotype C4 in Nasopharyngeal Swab Specimens

Kai Nie ,

Contributed equally to this work with: Kai Nie, Shun-xiang Qi, Yong Zhang

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Shun-xiang Qi ,

Contributed equally to this work with: Kai Nie, Shun-xiang Qi, Yong Zhang

Affiliation Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention of Hebei, Shijiazhuang, Hebei, People's Republic of China
⨯
Yong Zhang ,

Contributed equally to this work with: Kai Nie, Shun-xiang Qi, Yong Zhang

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Le Luo,

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Yun Xie,

Affiliation Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention of Hebei, Shijiazhuang, Hebei, People's Republic of China
⨯
Meng-jie Yang,

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Yi Zhang,

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Jin Li,

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Hongwei Shen,

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯
Qi Li ,

* E-mail: maxj2004@yahoo.com.cn (XJM); liqinew@yahoo.com.cn (QL)

Affiliation Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention of Hebei, Shijiazhuang, Hebei, People's Republic of China
⨯
Xue-jun Ma

* E-mail: maxj2004@yahoo.com.cn (XJM); liqinew@yahoo.com.cn (QL)

Affiliation State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China
⨯

Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction for the Detection of Human Enterovirus 71 Subgenotype C4 in Nasopharyngeal Swab Specimens

Kai Nie,
Shun-xiang Qi,
Yong Zhang,
Le Luo,
Yun Xie,
Meng-jie Yang,
Yi Zhang,
Jin Li,
Hongwei Shen,
Qi Li

Published: December 18, 2012
https://doi.org/10.1371/journal.pone.0052486

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